Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Effects of low-serum adaptation on growth and protein expression in stably lactoferrin-overexpressing mammary alveolar cells

doi: 10.3389/fbioe.2026.1731548

Figure Lengend Snippet: Immunofluorescence images of MAC-T cells. (A) Nucleus staining of MAC-T cells; (B) Cytokeratin 18 (CK18) staining; (C) Combined nucleus and CK18 staining. KSR: Knockout™ serum replacement. ITS: insulin–transferrin–selenium. Scale bar = 200 μm.

Article Snippet: After washed with pre-cooled PBS for 5 min, cells were fixed with 4% paraformaldehyde for 20 min, permeabilized with 0.1% Triton-X-100 for 1 h, and blocked with 5% BSA at room temperature for 2 h. Then primary antibody CK18 (Bioss, bs2043R) diluted at 1:100 was added, followed by overnight incubation at 4 °C and incubation with a fluorescent secondary antibody (Proteintech, RGAR004) at 1:500 dilution in the dark for 2 h. The cells were washed thrice with PBS and stained with DAPI (Beyotime, C1106) ( ).

Techniques: Immunofluorescence, Staining, Knock-Out

Journal: PLoS ONE

Article Title: The Use of a New CellCollector to Isolate Circulating Tumor Cells from the Blood of Patients with Different Stages of Prostate Cancer and Clinical Outcomes - A Proof-of-Concept Study

doi: 10.1371/journal.pone.0158354

Figure Lengend Snippet: The CTCs were identified and enumerated via a) positive nuclear staining (Hoechst), b) positive cytokeratin staining, and c) negative CD45 staining. d) Overlay of all images showing size and morphological characteristics.

Article Snippet: Subsequently, the cells were blocked with 3% bovine serum albumin (BSA) (PAA) in PBS for 1 h. Primary antibodies, including anti-pan-cytokeratin-fluorescein isothiocyanate (FITC) (CK8, CK18 and CK19, Abcam) and anti-CD45- allophycocyanin (APC, Invitrogen), were added for 1 h. The wire was then rinsed 3 times with 3 ml of PBS, and the nuclei were counterstained with Hoechst 33258 (Invitrogen).

Techniques: Staining

Journal: Oncotarget

Article Title: Glucocorticoid receptor antagonism promotes apoptosis in solid tumor cells

doi: 10.18632/oncotarget.27989

Figure Lengend Snippet: ( A ) In the MIA PaCa-2 model, efficacy of the combination of paclitaxel + relacorilant was significantly better than paclitaxel alone (non-parametric T -test P < 0.0001). ( B ) In the MIA PaCa-2 model, efficacy of paclitaxel + gemcitabine + relacorilant was better than paclitaxel + gemcitabine alone (non-parametric T -test P = 0.0005). ( C ) In the HeLa model, efficacy of paclitaxel + relacorilant was significantly better than paclitaxel alone (non-parametric T -test P < 0.0001). ( D ) In the CC6279 model, efficacy of paclitaxel + relacorilant was significantly better than paclitaxel alone (non-parametric T -test P < 0.0001). Relacorilant alone had no significant effect on tumor growth. Error bars represent the standard error; all studies 10 animals/group. ( E ) Tumor cells were labeled using cytokeratin 18 immunohistochemistry (top). In serial sections, apoptotic caspase activity (cleaved caspase 3, bottom) and proliferation (Ki67, not shown) were assessed. ( F ) Relacorilant increased the cleaved caspase intensity and prevalence (H-score) within the tumor cells compared to paclitaxel alone. *** Mann-Whitney, P < 0.0001. Abbreviation: IHC, immunohistochemistry.

Article Snippet: Primary antibodies included CK18 (EPR17347, Abcam), Ki67 (SP6, Abcam or 12202S, Cell Signaling Technologies), and cleaved-caspase-3 (9664s, Cell Signaling Technologies) and were all of rabbit origin.

Techniques: Labeling, Immunohistochemistry, Activity Assay, MANN-WHITNEY

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Preventive effects of transplantation of oral mucosal epithelial cells seeded on a decellularized amniotic membrane in a model of intrauterine adhesion

doi:

Figure Lengend Snippet: Immunohistochemical staining of CK-18 and HMW-CK in the dHAM+OMECs group. Endometrial epithelium and endometrial glands were positive for CK-18. However, 14 and 28 days after surgery in the dHAM+OMECs transplantation group, the regenerated epithelium was also observed and was positive for CK-18. Immunohistochemical staining of HMW-CK also revealed a positive expression of OMECs, but not endometrium and endometrial glands in the dHAM+OMECs transplantation group.

Article Snippet: The slices were immersed in 3% hydrogen peroxide solution for 15 min to block endogenous peroxidase before being incubated overnight at 4°C with primary antibodies including anti-cytokeratin 18 (CK-18) (bsm-33103M, Bioss) and anti-high molecular weight cytokeratin (anti-HMW-CK) (bs-1494R, Bioss).

Techniques: Immunohistochemical staining, Staining, Transplantation Assay, Expressing

Journal: Frontiers in Immunology

Article Title: A novel oral formulation of the melanocortin-1 receptor agonist PL8177 resolves inflammation in preclinical studies of inflammatory bowel disease and is gut restricted in rats, dogs, and humans

doi: 10.3389/fimmu.2023.1083333

Figure Lengend Snippet: Representative colon immunohistochemistry for K18 sections of DSS colitis-induced rats. Sham (A) is no challenge and no treatment. Placebo/vehicle control (B) is no treatment but DSS challenge. (C) is DSS challenge and treatment with PL8177 (100 µg). (D) is DSS challenge and treatment with mesalazine. (E) Quantification of positive K18 cells in colon crypts versus total crypt cells. Notes: material was limited for sham staining resulting in 2 colon tissues. Bar chart shows mean, SEM, and data points. DSS, dextran sulfate sodium; K18, cytokeratin 18.

Article Snippet: For immunohistochemistry, colon sections were processed for antigen retrieval and immunostained with primary antibody: keratin 18 (K18) (1:400, Bioss, bs-5405R) and with a rabbit-specific biotin-conjugated secondary antibody from HistoWiz (HistoWiz, Inc. Brooklyn, NY).

Techniques: Immunohistochemistry, Control, Staining